Satoshi NUMATA, Assistant Professor
1. Current Courses
Food Service Management training, On-site Clinical Practice
2. Research Area
Food Service Management
3. Research Topic
Development of highly sensitive enzyme immunoassay for proteins
4. Research Summary
I have been developing a highly sensitive enzyme immunoassay for proteins. The detection sensitivity of the highly sensitive enzyme immunoassay has reached a maximum value of zeptomole (1×10-21 mol; human ferritin). Highly sensitive enzyme immunoassay can be used to measure small amount of allergens in food and small amounts of proteins (hormones and antibodies) in urine.
5. Research Description
(1) I have been developing a highly sensitive enzyme immunoassay for insulin autoantibodies that is one of the diagnostic indexes for type 1 diabetes mellitus. The detection of insulin autoantibodies serum levels using highly sensitive enzyme immunoassay was found to be 1,000-fold more sensitive than using conventional ELISA. In addition, highly sensitive enzyme immunoassay was able to detect more insulin autoantibodies in serum from type 1 diabetes mellitus and type 2 diabetes mellitus than conventional ELISA.
(2) I plan to develop a highly sensitive enzyme immunoassay for the papain contained in the meat conditioners and tenderizers. The purpose of this study is to investigate the amount of permeation of papain in meat that has been treated with such conditioners, using highly sensitive enzyme immunoassay.
